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Individual Participant Data Meta-Analysis
The BMJ

Vitamin D Supplementation Reduces Acute Respiratory Infections: Meta-Analysis Evidence

A comprehensive meta-analysis of 25 randomized controlled trials involving 11,321 participants provides the strongest evidence yet that vitamin D supplementation safely reduces the risk of acute respiratory infections, with protective effects strongest in those with baseline deficiency.

February 15, 2017

Core Finding

Vitamin D supplementation reduced the risk of acute respiratory infection by 12% overall (OR 0.88, p<0.001). Participants with baseline vitamin D deficiency (25(OH)D <25 nmol/L) experienced a 70% greater risk reduction compared to those with sufficient baseline levels.

The Vitamin D-Immunity Connection

Vitamin D has long been recognized for its role in calcium metabolism and bone health. However, over the past two decades, research has revealed that vitamin D receptors are expressed on nearly all immune cells, including T cells, B cells, macrophages, and dendritic cells. This discovery sparked interest in vitamin D as a modulator of immune function.

The cathelicidin antimicrobial peptide—a key component of innate immunity—is directly upregulated by vitamin D. When immune cells encounter pathogens, vitamin D receptor activation triggers production of cathelicidin, which disrupts bacterial cell membranes and enhances viral clearance. This mechanism provides biological plausibility for vitamin D's role in respiratory defense.

Before this meta-analysis, individual trial results had been inconsistent, with some showing benefit and others showing no effect. The variation was likely due to differences in dosing regimens, baseline vitamin D status of participants, and outcome definitions. This study used individual participant data (IPD) meta-analysis, the gold standard for synthesizing clinical trial evidence, to resolve these uncertainties.

Study at a Glance

Study Overview

Source: The BMJ (2017)

Design: Individual Participant Data Meta-Analysis of 25 RCTs

Sample: 11,321 participants (ages 0-95)

Intervention: Vitamin D² or D³, various doses and regimens

Comparison: Placebo or no intervention

Primary Outcome: Incidence of acute respiratory infection

  • Overall effect: 12% reduction in respiratory infections (OR 0.88, 95% CI 0.81-0.96)
  • Deficient participants: 70% greater benefit when baseline 25(OH)D <25 nmol/L
  • Daily/weekly dosing: Consistent protective effect (OR 0.81)
  • Bolus dosing: No significant protection (OR 1.02)
  • Age effect: Benefits observed across all age groups
  • Safety: No serious adverse events attributed to supplementation

The protective effect was most pronounced in those receiving daily or weekly supplementation rather than large intermittent bolus doses, suggesting that steady-state vitamin D levels are important for immune function.

Clinical Implications

The findings from this meta-analysis support several important clinical applications:

1. Testing and Treatment Strategy

For individuals with recurrent respiratory infections, checking 25-hydroxyvitamin D levels is reasonable. Those with levels below 50 nmol/L (20 ng/mL) may benefit from supplementation. The optimal target for immune function appears to be 100-150 nmol/L (40-60 ng/mL), higher than the minimum threshold for bone health.

2. Prevention in High-Risk Groups

The study found particularly strong effects in:

  • Elderly individuals with baseline deficiency
  • Asthmatics and those with chronic obstructive pulmonary disease
  • Frequent infection sufferers (≥4 respiratory infections per year)

These groups should be prioritized for vitamin D testing and potential supplementation.

3. Public Health Considerations

Given the safety profile and low cost of vitamin D supplementation, there is a case for population-level approaches in regions with high deficiency prevalence. However, blanket supplementation without testing may miss those who need it most while unnecessarily treating others.

Important Considerations

  • Vitamin D toxicity is rare but possible with prolonged excessive dosing (>10,000 IU/day)
  • Individuals with sarcoidosis, granulomatous diseases, or certain lymphomas may hyper-metabolize vitamin D and require monitoring
  • Vitamin D interacts minimally with most medications but can affect certain anti-epileptics and glucocorticoids
  • Sun exposure contributes to vitamin D status but varies dramatically by latitude, skin pigmentation, and season

Practical Recommendations

Based on the evidence, a practical approach for most adults:

Testing and Dosing Recommendations

Unknown Status Consider 1000-2000 IU/day of vitamin D³ for maintenance

Baseline Testing Check 25(OH)D if risk factors for deficiency exist

Deficient (<25 nmol/L) 4000-6000 IU/day for 8-12 weeks, then recheck

Insufficient (25-50 nmol/L) 2000-4000 IU/day, recheck in 3 months

Sufficient (>50 nmol/L) 1000-2000 IU/day for maintenance

Optimal Target 100-150 nmol/L (40-60 ng/mL)

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Vitamin D Supplementation Reduces Acute Respiratory Infections: Meta-Analysis Evidence | Paper Interpretation