Vitamin LevelsUpdated 2025-12-24Medically Reviewed
Vitamin D (25-OH)
Everything you need to know about Vitamin D (25-OH) levels, including what normal ranges look like and what abnormal results might indicate.
Standard Reference Range
Unit: ng/mLMale Reference Range
30–100 (sufficient) ng/mL
Female Reference Range
30–100 (sufficient) ng/mL
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Reference ranges may vary slightly between laboratories. Always consult your doctor for interpretation.
What is Vitamin D (25-OH)?
The 25-hydroxy vitamin D level reflects total vitamin D stores from sunlight, diet, and supplements. It is the best screening test for deficiency.
Key Takeaway
Levels under 20 ng/mL indicate deficiency; 20–29 ng/mL is insufficiency; 30–50 ng/mL is generally adequate.
Why is this test performed?
- Evaluate bone health and osteoporosis risk
- Investigate low calcium/phosphorus or high PTH
- Monitor supplementation therapy
Interpreting Your Results
- Low: Poor sun exposure, malabsorption, kidney/liver disease, limited diet
- High: Excess supplementation; rare granulomatous diseases can produce excess active vitamin D
Related Tests
- Calcium/Phosphorus: Balance with vitamin D
- PTH: Rises when vitamin D or calcium is low
- Magnesium: Needed for vitamin D metabolism
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